This article presents the results of research that explores the relationship between parenting practices, children’s temperament and their prosocial behavior. The sample consisted of 281 parents (M = 40.1 years and SD = 7.0) with children (M = 11.3 years and SD = Three instruments were used: Parenting Practices Questionnaire (CPC-P) - parent version, Youth Temperament and Character Inventory (JTCI) - parent version and Prosocial Behavior Scale for adolescents. Logistic regression was used for the analysis of results, which show that the association between the variables of parenting practices and child temperament predict children’s prosocial behavior.