During the search of the Cervantes’s remains in 2015, a sample of 450 subadult skeletons was exhumed from the crypt of the Trinitarias Church in Madrid. It was observed that in those remains dated from the last XVIIIth to the XIXth century, there was a high frequency of paleopathological indicators of rickets or vitamin D deficit (>60% of the individuals). Regarding to the dental pathologies that were found in the sample, 12 cases with serious amelogenesis alterations (Hypocalcification and hypoplasia) are presented in individuals from 0 to 3 years old. Amelogenesis disruptions when isolated can be due to local infections or traumatisms, while when they are generalized they can be a consequence of any metabolic disorder or nutritional deficiency. The enamel anomalies of each tooth from these 12 individuals were classified in 6 groups according to the FDI criteria. Furthermore, the location of the disruptions was studied in order to estimate the age in which the event took place. Regarding to the results, the highest frequency of hypoplasia was classified into type 2 and type 6. In each individual several episodes are observed which probably means that the metabolic disorder occurred in different periods of growth. This sample is one of the most important discoveries in the study of palaeopathology in subadults due to the severity of signs consistent with rickets and the hypoplasia described in the present study.